Composite
Part:BBa_K2994001:Design
Designed by: Qitong Luan Group: iGEM19_Gaston_Day_School (2019-10-06)
Temperature Sensitive GFP Production Construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
J23100 is a strong constitutive promoter, and B0035 is strong RBS. The combination of them increases the efficiency for the production of gTEMP.
Source
Synthetic construct of a combination of Sirius and mT-Sapphire
References
Nakano, M. Arai, Y. Kotera, I. Okabe, K. Kamei, Y. Nagai, T. (2017). Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLOS ONE, 12(2), e0172344. https://doi.org/10.1371/journal.pone.0172344